HPLC Column, Hyper Similars C8 Octyl, 3um, 4.0mm ID x 250mm Length, 150A. Cogent Brand. 1 EA. Special Order Item.

Additional Information:

Hyper Similars C8™ Octyl is a traditional reversed-phase HPLC stationary phase designed for laboratories seeking a reliable alternative to established C8 methods and legacy column platforms. Part of the Cogent™ Similars product line, it is manufactured using high-purity Type-B spherical silica with a bonded octyl (C8) ligand and full endcapping for dependable chromatographic performance. The silica surface characteristics closely resemble those found in Thermo Fisher Hypersil™ and Spherisorb® columns, making this USP L7 stationary phase well suited for method transfer, validation, and replacement of discontinued C8 columns while maintaining familiar retention and selectivity characteristics.

62308-25P-4 is a 250 mm x 4.0 mm ID analytical column packed with 3 µm, 150Å Hyper Similars C8 particles. The combination of a longer column length and smaller particle size provides high efficiency, increased theoretical plate counts, and enhanced resolving power for difficult separations. This configuration is particularly useful for impurity profiling, pharmaceutical assays, and complex sample matrices where maximum resolution is preferred over shorter run times. The 4.0 mm ID format is commonly selected for reproducing legacy methods, while typical operating flow rates are approximately 0.8–1.5 mL/min on conventional HPLC systems. As with most 3 µm columns, higher operating backpressure should be anticipated compared to 5 µm packings.

This column is ideal for hydrophobic and neutral compounds analyzed by reversed-phase HPLC when less retention is desired than a comparable C18 phase. Common uses include pharmaceutical analysis, environmental testing, long-chain fatty acid separations, quality control, and method development. This product is supplied as a Special Order Item manufactured specifically upon receipt of order.

Key Benefits

  • Type-B spherical silica stationary phase

  • Suitable alternative for USP L7 methods

  • Comparable selectivity to Hypersil® and Spherisorb® C8 columns

  • Increased resolution from 250 mm column length

  • Higher efficiency with 3 µm particles

  • Sharper peaks and improved separation performance

  • Less retention than C18 columns

  • End-capped octyl (C8) bonded phase

Typical Applications

  • USP L7 methods

  • Reversed-phase HPLC

  • Pharmaceutical analysis

  • Impurity profiling

  • Method development

  • Long-chain fatty acid analysis

  • Environmental testing

  • Method transfer and validation

Specifications

  • Product Type: HPLC Column

  • Stationary Phase: Hyper Similars C8 Octyl

  • USP Classification: L7

  • Particle Size: 3 µm

  • Particle Type: Type-B Spherical Silica

  • Bonded Phase: C8 (Octyl)

  • Endcapped: Yes

  • Pore Size: 150Å

  • Column Dimensions: 4.0 mm ID x 250 mm

  • Length: 250 mm

  • Recommended Flow Rate: 0.8–1.5 mL/min

  • Brand: Cogent

Special Order Item Information

This item is a Special Order Item manufactured specifically to customer requirements.

Important Notes

  • Designed for reversed-phase chromatography

  • Longer column length provides increased resolving power

  • 3 µm particles provide higher efficiency and sharper peaks

  • Higher backpressure than comparable 5 µm columns

  • Compatible with conventional HPLC systems

  • Provides less retention than comparable C18 stationary phases

  • Special Order Item


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